FARM MINISTER: THE WHOLE EUROPEAN UNION SHOULD BE A GMO-FREE ZONE

Source: http://hungarytoday.hu/cikk/farm-minister-whole-european-union-gmo-free-zone-67084

Hungary will initiate a joint alliance of European Union member states rejecting the use of genetically modified organisms in farming with the aim to make the entire European Union free from GMO crops, Hungarian farm minister Sándor Fazekas said on the sidelines of the Global Forum for Food and Agriculture in Berlin at the weekend. István Fazekas said the countries committed to GMO-free farming must forge an alliance because “we would like to keep our food and our environment healthy and we are convinced that the agriculture of the EU and Hungary can remain competitive with conventional crop and livestock production”.

Farm Ministry said last week that Hungary’s interests were fulfilled when a vote passed in the European Parliament allowed individual member states to ban the cultivation of GM crops. “It is an especially important strategic interest for Hungary laid down in its constitution also to ensure that its agriculture is GMO-free,” it said. During talks which lasted over four years Hungary had always urged a solution which ensures an effective tool for imposing a ban on GMO. The law was supported by 480 MEPs with 159 votes against and 58 abstentions.

As newportal Euractiv.com reported, pro-GM governments, such as the UK and the Netherlands, had wanted to see many more varieties approved and growing in their soils. But they were frustrated by opponents, such as France, Germany, Luxembourg and Austria, which blocked the qualified majority required in Brussels to give the go-ahead. These countries together with Bulgaria, Greece, Hungary, Poland and Italy have adopted safeguard measures prohibiting the cultivation on their territories. Since last week member states are allowed to restrict or prohibit the cultivation of specific GMOs in their territory even if Brussels gives the green light for their cultivation.

via hungarymatters.hu, MTI and euractiv.com photo: nol.hu

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Le rapport caché de l’OMS sur les crimes US en Irak

Algérie Résistance

Birth-Defects-004Les crimes monstrueux de l’armée US et britannique en Irak. D.R.

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C’est un scandale planétaire que l’on pourrait nommer l’OMSgate. Depuis novembre 2012, l’OMS (Organisation Mondiale de la Santé) bloque la parution de son rapport sur les effets dévastateurs des bombardements à l’uranium appauvri (UA : déchet radioactif issu de l’enrichissement de l’uranium destiné aux réacteurs civils et militaires) sur la santé de la population irakienne. En effet, suite à la parution fin 2014 du classement Censored 2015, programme universitaire répertoriant les 25 informations les plus importantes au niveau mondial et censurées par la presse, il apparait que l’OMS a censuré son propre rapport établi avec l’aide du Ministère irakien de la Santé concernant…

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Prayer and Meditation for Monday, January 12, 2015 — “The time has come and the Kingdom is close at hand. Repent and believe in the Good News.”

Peace and Freedom

Monday of the First Week in Ordinary Time
Lectionary: 305

Jesus-Follow Me and I will make you fishers of men

Jesus said, “Follow Me and I will make you fishers of men.” By Alex Acropolis Calderon

Reading 1 Heb 1:1-6

Brothers and sisters:
In times past, God spoke in partial and various ways
to our ancestors through the prophets;
in these last days, he spoke to us through the Son,
whom he made heir of all things
and through whom he created the universe,who is the refulgence of his glory,
the very imprint of his being,
and who sustains all things by his mighty word.
When he had accomplished purification from sins,
he took his seat at the right hand of the Majesty on high,
as far superior to the angels
as the name he has inherited is more excellent than theirs.

For to which of the angels did God ever say:

You are my Son; this day I…

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False Flag Terror—The Occult Connections

William Ramsey looks at the entire sequence of the bombing events of the last decade and decodes the occult connections.
SOURCE: http://www.henrymakow.com/false_flag_terror—the_occult.html

by William Ramsey

(for henrymakow,.com)

During the imperial Bush presidency, many ostensibly terrorist-inspired bombings took place throughout the world. In particular, there were attacks in the following cities:

New York, United States

Bali, Indonesia

Madrid, Spain

London, England

Amman, Jordan

These attacks shocked the world and convinced the global populace that a “War on Terror” was necessary to ensure their safety. However, upon investigation, many of the so-called terrorist bombings and/or attacks were revealed to be false flag operations. These false flag operations seem to have the intent to propel a covert agenda of global war and globalization. Does this secret agenda have an even darker connection to the occult? Do the dates of these attacks have particular meaning in the occult?

PROPHET OF THE NEW AGE

Aleister Crowley, was born October 12th, 1875 into a wealthy family in Warwickshire, England. Crowley (rhymes with holy) rebelled against the Christian morality of his parents and committed himself to a life of occultism. A well-educated and talented litterateur, Crowley wrote many volumes of occult works detailing his dark vision of the future. He believed that, “despite all its drawbacks, there was never a better social system than the feudal, so far as it derived from the patriarchal.”[1] He also stated that the government must lord over and manage hordes of ignorant masses:

We should have no compunction in utilizing the natural qualities of the bulk of mankind. We do not insist on trying to train sheep to hunt foxes or lecture on history; we look after their physical well-being, and enjoy their wool, and mutton. In this way, we shall have a contented class of slaves who accept the conditions of existence as they really are, and enjoy life with the quiet wisdom of the cattle.[2]

Is there a connection between Crowley and the false flag terror events that has been overlooked? Is there a deeper meaning to the dates of the terror events? Why are those numbers relevant to Crowley?

The number 11 is the number most important to Aleister Crowley. He learned of the symbolic meaning of the number from his research into the occult in the later part of the nineteenth century. As a member of the quasi-Masonic magical group The Hermetic Order of the Golden Dawn, he was exposed to writings of the groups founders. William Wynn Westcott, one of the original organizers of the Golden Dawn, refers to the number eleven in his book Numbers: Their Occult Powers and Mystic Virtue:

“Upon the Number Eleven. This seems to have been the type of number with a evil reputation among all peoples. The Kabbalists contrasted it with the perfection of the Decad, (10) and just as the Sephirotic number is the form of all good things, so eleven is the essence of all that is sinful, harmful and imperfect; with the Ten Sephiroth they contrasted the Eleven Averse Sephiroth, symbols of destruction, violence, defeat, and death.”[3]

Aleister Crowley wrote about the number 11 frequently, and emphasized it’s importance as shown below:

“Firstly”, 11 is the number of Magick in itself. It is therefore suitable to all types of operation. “Secondly”, it is the sacred number par excellence of the new Aeon. As it is written in the Book of the Law: …11, as all their numbers who are of us.”[4]

Crowley’s much repeated phase “Do What Thou Wilt Shall Be The Whole Of The Law” (which he considered the dictum of the New Age), has eleven words and eleven syllables. Based on the preceding information, eleven is a crucial number in Crowley’s religious system.

The number 77 also has an important meaning to Crowley and his followers. According to Crowley, 77 is the:

…sublime and supreme septenary in its mature magical manifestation through matter…written in Hebrew Ayin Zayin (OZ)…Capricornus, the Devil of the Tarot; which is the picture of the Goat of the Sabbath on an altar.[5]

77 also represents half of the formulation of sexual magic in both the O.T.O. and the Kabbalah.[6] It also refers to the 77 infernal names of the Devil in book The Satanic Bible by Anton LaVey.[7]

88 is an important number for what can be termed the Illuminati. It refers to number of squares high and wide on a chess board, a symbol of the capacities of the human mind.[8]

10/12 is an occult feast celebrating the birth of the Prophet of the New Aeon–Aleister Crowley.

Thus, 11, 77 and 88, and 10/12, the satanic feast for Aleister Crowley’s birthday, called Crowleymas, are significant numerical markers for the occult practitioner. Did each of these terror attacks have an occult numerical marker? Were the attacks situated on certain dates for occult reasons?

Here are the following events in the phony “War on Terror” with occult, numerical significance:

10/12 (2000)—U.S.S. Cole Bombing. A false-flag terrorist attack against the United States Navy destroyer U.S.S. Cole on October 12, 2000 while it was harbored and refueling in the Yemeni port of Aden. 17 American sailors were killed, and 39 were injured. The attack occurred on Crowleymas, Aleister Crowley’s 125th birthday.

9/11 (2001)—9/11. The false-flag September 11 attacks used four commercial passenger jet airliners. Two of the airplanes, Flights 11 and 175, crashed into the Twin Towers of the World Trade Center in New York City, killing everyone on board and many others in the buildings. Both buildings imploded within two hours. A third airplane, Flight 77, crashed into the Pentagon in Arlington, Virginia. The fourth airplane, Flight 93, crashed (or was shot down) near Shanksville in rural Pennsylvania. The attacks killed 3,000 people.

10/12 (2002)—Bali Bombings. The false-flag Bali bombings occurred on October 12th, 2002 in the tourist district of Kuta on the Indonesian island of Bali. The coordinated attack involved the detonation of three bombs: a backpack-mounted device carried by a suicide bomber; a large car bomb, both of which were detonated in or near popular nightclubs in Kuta; and a third much smaller device detonated outside the United States consulate in Denpasar, causing only minor damage. The bombings killed 202 people and injured 240. The bombings occurred on Crowleymas, Aleister Crowley’s 127th birthday.

3/11 (2004)—Madrid bombings. The false-flag Madrid train bombings consisted of a series of coordinated bombings against the commuter train system of Madrid, Spain on the morning of March 11th, 2004 The Madrid bombings occurred three days before Spain’s general elections. The bombings killed 191 people and roughly 1,800 injured. The bombing occurred 911 days after the September 11th attacks.

7/7 (2005)—London “7/7” bombing. The false-flag London bombings (often referred to as 7/7) were a series of coordinated suicide attacks upon London’s public transport system. Four unwitting patsies were guided to locations in the London Underground. where three bombs were exploded. A fourth bomb exploded an hour later on a red double-decker bus in Tavistock Square. The bombings killed 52 people and roughly 700 were injured.

11/9 (2005)—Amman, Jordan bombing. The false-flag bombings were a series of coordinated bomb attacks on three hotels in Amman, Jordan, on November 9, 2005. The bombings killed 60 people and injured 115 others.

8/8 (2008)—an attempted U.S. war with Russia, using Georgia as a proxy. During the night of August 8th, Georgia launched a large-scale military attack against South Ossetia, in an attempt to conquer the territory. The following day Russia reacted by deploying combat troops in South Ossetia and launching bombing raids deep into Georgia.

Clearly, these major terrorist bombings were situated on dates containing the numbers 11, 77, 88 and Aleister Crowley’s birthday. Based on the numerical significance of the dates of these false-flag terror events, an occult correlation can be made. When the bombings are listed together, one can clearly discern the hidden, occult agenda for war and greater global control. A detailed analysis of Crowley’s connection to these events is contained in my book Prophet of Evil: Aleister Crowley, 9/11 and the New World Order, available on Kindle and at my website: http://www.occult911.com.

William Ramsey

Author: Prophet of Evil: Aleister Crowley, 9/11 and the New World Order

Writer and Director of the new film: Prophet of Evil: Aleister Crowley http://www.occult911.com

occult911@gmail.com

[1] Crowley, Aleister, John Symonds, and Kenneth Grant. The Confessions of Aleister

Crowley: an Autohagiography. London: Arkana, 1989. 566. Print.

[2] Crowley, Aleister, Louis Wilkinson, Hymenaeus Beta, and Aleister Crowley. The Law Is for All: the Authorized Popular Commentary on Liber Al Vel Legis Sub Figura CCXX, the Book of Law. Phoenix, Ariz.: New Falcon, 1996. 199-200. Print.

[3] Westcott, W. Wynn, Numbers: Their Occult Power and Mystic Virtue. Being a Resume of the views of the Kabbalists, Pythagoreans, Adepts of India, Chaldean Magi and Medieval Magicians Theosophical Publishing Society, London 45 Print.

[4] Crowley, Aleister Magick: in Theory and Practice. Secaucus, NJ: Castle, 1991. 64. Print.

[5] Crowley, Aleister. The Book of Lies, Which Is Also Falsely Called Breaks: the Wanderings or Falsifications of the One Thought of Frater Perdurabo (Aleister Crowley), Which Thought Is Itself Untrue. Boston, MA: Weiser, 2001. 185. Print.

[6] Grant, Kenneth. Aleister Crowley & the Hidden God. Kuala Lumpur: Skoob Books, 1993. 196. Print.

[7] Gilmore, Peter H., and Anton Szandor Lavey. Satanic Bible. Reissue ed. Boston: Avon, 1969. pp. 44-6. Print.

[8] The Law of Octaves was first suggested by Pythagoras in ancient Greece. Having observed that the eight notes of the conventional Occidental musical scale were governed by definite mathematical relationships, Pythagoras proceeded to create a whole cosmology based on 8s. In this octagonal model Pythagoras made numerous mistakes, because he was generalizing from insufficient data. However, his work was the first attempt in history to unify science, mathematics, art and mysticism into one comprehensible system and as such is still influential. Leary, Crowley and Buckminster Fuller have all described themselves as modern Pythagoreans. In China, roughly contemporary with Pythagoras, the Taoists built up a cosmology based on the interplay of yang (positive) and yin (negative), which produced the eight trigrams of the I Ching, out of which are generated the 64 hexagrams. In India, Buddha announced, after his illumination under the Bodhi tree, the Noble Eightfold Path. Patanjali subsequently reduced the science of yoga to eight “limbs” or, as we might say, eight “steps.” The game of chess appeared, somewhere in the East, with a grid based on 8×8 (64) squares. Wilson, Robert Anton. Cosmic Trigger: Final Secret of the Illuminati. Tempe, AZ: New Falcon, 2007. 266. Print.

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Comments for “False Flag Terror—The Occult Connections”

Abe said (October 27, 2010):

Hey Henry, Found the article insightful but felt the need to point out that a chess board has 64 squares, not 88. Other points of interest are the chess themes that run through the pedophile originator of western children’s literature Lewis Carol’s “through the looking glass” and the fact that the number 88 is used in neo-nazi circles to mean “HH” or Heil Hitler. Perhaps these lead to further connections, but none that I’m aware of off the top.

William meant the squares by dimension but thanks for pointing out this error which he has acknowledged and clarified.

Mike said (October 26, 2010):

7/7 RIPPLE EFFECT Video http://youtu.be/R7PQG5weeHk

So sloppy & blatant…that it shows just how much contempt those at the top have for us sheeple. Ask questions people !!!
Marc said (October 26, 2010):

Jake Kotze first discovered and came up with what he calls Hollywood-Masonic “synchronicity” – he has many youtubes videos describing the occult signatures of 9/11: 9/11 Stargate, 9/11 Time Lords across the 4rth dimension, Inside job/synchronicity 9/11, The 9/11 Pyramid Mega-Ritual,The 9/11 Mega Ritual. He describes it as cosmic conscienceness synchronicity, which of course I disagree, I call it occult conspiracy,which has it’s tentacles in high corridors of power.

James said (October 26, 2010):

With regard to commenter Francois, Crowley’s creed is being lived by in large part by modern man, the society of immoral hedonism benefits
none, save the “leaders” and is merely an opiate of the people, Crowley was well aware of this, for a drugged herd is easy to control, and the controllers use the animals for the labors and tasks they are too lazy and self-important to accomplish, their existence becomes easy and luxurious, and the animals are reduced to living in foetid stables, that is the sum of crowley’s concept and ideals. Clearly, not a good ideal to follow, and in fact a very negative and vile one.

Even his kabbalistic numerology and gematria reflect this, for the numbers cited as important, not only are, but represent imbalance and
excess (11 does not break equally, neither do the later numbers, each break into flawed fractions, emblems of imperfection and disorder
all.) Should I even bother to point out that with the simplest new agey numerological method, that 175 (1+7+5) equals 13? (and any two-bit hobby-occultist knows the signifigance of *that* number.)

taking it a step further results in 4, a number held by the Japanese to be on par with the western 13, and a symbol of death.

All that aside however, Don’t be alarmed Rod, Henry knows what he’s doing, and exposing the methods of their madness doesn’t mean that
someone will go out and try the stuff, as it seems the majority of the readers wouldn’t be of the mentality to blunder into something so
foolish. To Mr. William I extend a thanks for calling attention to these number peculiarities, and to you Henry, thanks as well, for not
being afraid to show such things, After all to defeat the beast, one must first find it’s tracks, then one may be able to find it and slay
it in it’s den, instead of wandering the woods to be leapt upon unawares, again and again.

S said (October 26, 2010):

Soooooo, if Crowley was so damn important and powerful why did he end up dying a drug-ravaged old man on the government dole?

Dear S.

Because he was bad.

Rod said (October 26, 2010):

Yes , all the sick bastards who were complicit to the 9/11 murders are probably proud satanists and practice or admire the occult. But PLEASE, do we have to continually read the b/s “hijacked planes crashed” stories ?? “If” they were indeed hijacked they certainly did NOT fly them into the Twin Towers or the Pentagon. There’s enough video evidence by just the mainstream media alone to show that airplanes didn’t hit any of the 3 buildings. Big lies and make bigger fools of any that still believe them. And do we really have to promote/teach occult in your forums ?? Who the hell cares how these sickos practice conjuring up demons or what they do with there damn numbers ? So are you just going to continue to promote evil by telling people how to do it, or will you take a stand on the side of truth and what’s right, and quit pretending that it’s ok to show evil in the same light as good ? How about focusing on how to fight this new world order crap instead of just pointing at them like saying “look how well they are doing you stupid sheep”. Enough is enough Henry. Show your true colors. Or are you showing them and I just don’t want to believe it ? I hope not. For the readers’ sake and your own.

FRANCOIS said (October 26, 2010):

don’t really see how these Crowley’s quotes may be seen in such a negative light.

Really read what is written and what it implies, putting the we aside. Wouldn’t we be enjoying life if we were contented ? We are currently nowhere treated as well as cattles. Sheeps don’t pay rents or mortgage, sheeps are fed. Too many humans are homeless, too many humans are led to starvation.

And his famous motto “Do What Thou Wilt Shall Be The Whole Of The Law”, when you think about it, is strictly followed by every specie on the planet, save one.

Wouldn’t our turmoils come from the simple fact that we cannot accept that we are beasts, animals, parts of nature ?

And similarly, I don’t see a direct link between Crowley and the current affairs. Numbers (and their properties) belong to everyone.

Henry Makow received his Ph.D. in English Literature from the University of Toronto in 1982. He welcomes your comments at henry@henrymakow.com

– See more at: http://www.henrymakow.com/false_flag_terror—the_occult.html#sthash.WPSm5TEg.dpuf

See more at: http://www.henrymakow.com/false_flag_terror—the_occult.html#sthash.WPSm5TEg.dpuf

CaMV 35S Promoter in GM Feed that Sickened Rats Transferred into Rat Blood, Liver, and Brain Cells

Source: http://www.i-sis.org.uk/CaMV_35S_Promoter_in_GM_Feed_that_Sickened_Rats.php

The rats were fed an ordinary rat chow found to contain GMOs on PCR analysis using probes for the cauliflower mosaic virus 35S promoter, a gene control element in more than 80 % of commercial GM crops grown with potential health hazards predicted since 1999 Dr Mae-Wan Ho

A fully referenced and illustrated version of this article is posted on ISIS members website and otherwise available for download here

Please circulate widely and repost, but you must give the URL of the original and preserve all the links back to articles on our website. If you find this report useful, please support ISIS by subscribing to our magazine Science in Society, and encourage your friends to do so. Or have a look at the ISIS bookstore for other publications

Researchers led by Hanaa Oraby at Egypt’s National Research Center in Cairo are not the first to look for horizontal transfer of genetically modified (GM) DNA into animal cells, but certainly among the first to do an experiment aimed at detecting it and succeeded [1]. Horizontal gene transfer is the direct uptake of DNA (or RNA) into cells and integration of the sequence into the cell’s genome. Some of us regard horizontal gene transfer as the most serious hidden hazard of genetically modified organisms (GMOs) released into the environment ([2] Horizontal Gene Transfer – The Hidden Hazards of Genetic Engineering (ISIS special report). But a prevailing culture of denial by vested interests and regulators has obstructed proper investigation until very recently (see [3] Horizontal Transfer of GM DNA Widespread, SiS 64).

A GMO is an organism with synthetic foreign DNA gene sequences inserted into its genome in a laboratory process of artificial genetic modification that bypasses normal reproduction. Part of the foreign DNA is a control element called a promoter that is necessary for expressing the foreign genes. The most widely used is the cauliflower mosaic virus (CaMV) 35S promoter (which is what enables the virus to hijack the cell for making endless copies of the virus). The CaMV 35S promoter is now in more than 80 % of all GM plants [4], and is the first test for the presence of GMOs in unknown samples.

Probing for CaMV 35S promoter in the rat diet and in rat tissues

The Cairo researchers used three pairs of primers – specific short anchoring sequences that bind by specific base-pairing to the opposite ends of the DNA segment of interest – so as to amplify different segments from the CaMV 35S promoter with PCR (polymerase chain reaction). The amplified segments can then be isolated and detected on electrophoresis. The primers together amplify nearly 80 % of the entire promoter sequence. The experimental diet was an ordinary lab chow containing 60 % yellow maize and 34 % soybean, but unlabelled as to whether it is GM or not. The presence of GM material in the diet was ascertained using PCR assay with the three pairs of primers, which all gave the expected positive results, indicating that the diet contained GM material (up to a maximum of 94 %, if both the soybean and maize were completely GM).

The experimental animals consisted of 29 male Wistar albino rats immediately after weaning (age three to four weeks), which were divided into two main groups. One was fed on the lab chow containing GM ingredients for three months, and were further divided into three subgroups of 5, 5, and 7 animals, euthanized after 30, 60, and 90 days. The other control group was fed on a balanced non-GM diet for the same period and euthanized at the end of the experiment.

The lab chow diet used all through this experiment gave positive results when screened with primers for CaMV 35S promoter. The expected 195 bp (base pairs) amplified product was detected in all samples of the GM diet, but was absent in the control diet made up with non-GM material to match the nutritional content of the GM diet. As further confirmation, the PCR product obtained from the GM diet was sequenced, and shown to have 100 % identity with the CaMV 35S promoter at nucleotide coordinate 7190-7380 of the CaMV on sequence alignment analysis using the GenBank database. It also showed 100 % sequence identity with a number of binary vectors used for transferring genes in the lab that also contain the CaMV 35S promoter.

PCR analysis of the different tissues showed amplified sequence segments of the expected sizes for the three pairs of primers – 70, 88 and 195 bp – in some of the DNA samples of blood, liver and brain of rats fed the GM diet after 30, 60 and 90 days. None of the three primers gave amplification product in DNA samples of tissues from the controls fed the non-GM diet.

The 195 bp segment amplified from DNA samples of liver and brain in rats fed GM diet was subjected to DNA sequencing, and comparison with GenBank database revealed 100 % identity with the CaMV whole genome at the same nucleotide coordinates 7190-7384 for the 35S promoter. Furthermore, it also showed 100 % identity with the PCR segment amplified from the GM diet, and with the binary vectors segments that are 100 % identical to the PCR product from the GM diet.

Feeding rats with GM diet for 30, 60, and 90 days increased the mean transfer frequency of GM target sequences significantly from 0 in the controls to 8 + 0.0000 %, 12.3 + 1.2018 % and 16.7 + 2.4529 % respectively. Thus, there is a cumulative effect with time of exposure.

Bearing in mind that the three primer pairs together amplify nearly 80 % of the entire CaMV 35S promoter, and in some animals, the three segments were all amplified in the same sample, it suggests that the whole CaMV 35S promoter may have been transferred into the genome of those animals. Considering that even a promoter containing only 46 bp of the 5’ sequence from the CaMV 35S promoter was previously reported to be sufficient for accurate initiation of gene transcription for gene expression [5], it is highly likely that the transferred CaMV35S promoter sequence would alter the activity of some genes in the host cell genome that may have harmful consequences (see later).

There was no significant difference in the rate of transfer into blood, liver, or brain tissues. Moreover, the frequency of uptake for the larger segments was greater than that for the smaller segments; thus, the transfer frequency of the 70 bp segment was the lowest at 1.09 + 0.4161 %, the 88 bp at 2.09 + 0.7318 %, and the 159 bp at 3.8 + 0.8069 %. This finding is consistent with previous researchers who postulated that the shorter the fragment, the lower the uptake efficiency [6].

GM fed rats suffered damages to liver, kidney and testis

In a separate report written by some of the same researchers in Cairo, the same GM diet was fed to identical rats in a post-market safety assessment of GMOs [7]. Biochemical, histopathological, and cytogenetic analyses on liver, kidney, and testis revealed that the GM diet fed for 30, 60 and 90 days suffered significant deleterious effects.

A total of 30 rats were fed the GM diet, 10 each for 30 days, 60 days and 90 days. The controls were on a wheat-based non-GM nutritionally matched diet for similar periods of time.

Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme activities were measured in blood serum as indicators of liver cell damage. Creatinine and uric acid levels were determined also in blood serum as indicators of kidney function. Malondialdehyde in liver cells resulting from lipid peroxidation is a biomarker of oxidative stress. In addition, specimens of liver, kidney and testis were dissected immediately after the rats were euthanized and sectioned for histopathological and histochemical investigations. Cytogenetic and DNA damage analyses were carried out on testis and liver. Chromosome analysis was done on germ cells from the testis. Sperms were examined for morphological abnormalities and DNA fragmentation was determined in liver cells.

The results were unambiguous.

Histopathology of liver, kidney and testis

Liver cells showed slight damage in rats fed GM diet for 30 days, with damage increasing after 60 and 90 days. The effects start as a slight dilatation and congestion of the central vein (supplying the liver) and fragmentation of the nucleus in some cells. After 60 days, mild cellular infiltration (from the blood) was observed. After 90 days, the blood sinusoids (spaces) also showed slight dilatation and congestion (Figure 1).

Figure 1 Liver sections: a, control; b, 30 day GM-fed; c, 60 day GM-fed; d 90 day GM fed (see text for details)

Kidney sections show damaging effects of the GM diet evident even after the first 3o days as interstitial haemorrhage (blood in spaces between cells) and a widening of the tubules. This got worse in 60 and 90 days (Figure 2).

Figure 2 Kidney sections: a, control; b, 30 day GM-fed; c, 60 day GM-fed; d 90 day GM fed (see text for details)

The testis of rats fed on GM food for 30 days showed mild thickening of the basement membrane of the seminiferous tubules (where germ cells develop) with gaps appearing between the germinal epithelium of some tubules. At 60 and 90 days, an increase in the connective tissue component and in the number of Leidig cells (in the connective tissue), with a disarrangement of the germinal epithelium (Figure 3).

Figure 3 Testis sections: a, control; b, 30 day GM-fed; c, 60 day GM-fed; d 90 day GM fed (see text for details)

Protein content in liver tissues decreased significantly after 30 or 90 days, indicating dysfunction of some hepatocytes. Abnormal cellular activity in the kidney was also confirmed by a statistically significant increase in the protein content.

Consistent with the liver and kidney damages seen, AST and ALT activity increased in the serum of experimental rats by 33 to 107 % and 33 to 92 % respectively. Blood creatine and uric acid concentrations significantly increased by 15 to 315 % and 37 to 96 % respectively. MDA concentrations in liver, as an indicator of oxidative stress, increased significantly in all animals fed GM diet by 286 to as high as 940%.

Mitotic index (as a measure of cell division) was significantly reduced in the 60 and 90 days fed rats from 8.8 + 0.326 % in controls to 8.4 + 0.221 % (30 days), 6.8 + 0.466 % (60 days) and 6.6 + 0.266 % (90 days). Concomitantly, there was a significant increase in chromosomal aberrations, from 0.4 + 0.163 % in controls to 6.6 + 0.221 %, 13.8 + 0.326 % and 8.0 + 0.632 % respectively for 30, 60 and 90 day GM fed rats. The frequency of morphologically abnormal sperm increased by up to two-fold, from 3.33 +0.35 % to 5.83 + 0.60 %, 7.8 + 0.65 % and 6.6 + 0.24 %. At the same time DNA fragmentation went up from 11.83 + 0.7 % in controls to 19.0 + 1.2 %, 28.3 + 1.6 % and 24.3+ 0.7% respectively.

The researchers concluded the results [7] “indicate that there are health hazards linked to the ingestion of diets containing genetically modified components.”

However, the investigations have not gone further into the mechanisms whereby the genetically modified components were hazardous to health; nor do the results directly implicate the CaMV 35S promoter found transferred to rat blood, liver and brain in the other report [1]. It will be useful to review the potential hazards of the CaMV 35S promoter, which were first pointed out 15 years ago.

Predicted hazards of cauliflower mosaic virus 35S promoter

When first deployed, geneticists assumed that the CaMV 35S promoter would only work in plants, as the complete virus (wrapped in its protein coat) specifically infects only plant cells. But it soon transpired that the isolated piece of promoter DNA without its coat is extremely promiscuous, and works in cells across kingdoms of plants and animals, as well as bacteria. We issued a serious warning against its use in 1999 [8] Cauliflower Mosaic Viral Promoter – A Recipe for Disaster (ISIS scientific publication) when it was found to have a recombination hotspot where it tends to fragment and join, which makes it prone to unintended (horizontal) gene transfer into cells of all organisms exposed to the GMO, including bacteria, fungi, pollinators, wild animals and humans (see [9] CaMV 35S promoter fragmentation hotspot confirmed, and it is active in animals (ISIS scientific publication). What that implies is the CaMV 35S promoter can break loose from the plant genome DNA and jump into the genome of all those other cells, with the potential to mutate, activate or inactivate genes (including those leading to cancer), reactivate dormant viruses, or create new viruses by recombination (gene shuffling) [8, 10] (Hazards of Transgenic Plants Containing the Cauliflower Mosaic Viral Promoter, ISIS scientific publication) But our warnings were met with abuse and denial and ultimately ignored.

Since then, evidence has emerged that the CaMV 35S promoter may enhance the multiplication of disease-associated viruses including HIV and cytomegalovirus through the induction of proteins required for transcription of the viruses [11] (New Evidence Links CaMV 35S Promoter to HIV Transcription, ISIS scientific publication). Further, the CaMV 35S promoter overlaps with a virus gene, the product of which is toxic to plants and likely also to animals [12]. For a more detailed description on the risks of the CaMV 35S promoter and indeed on GMOs in general, see [13] and final chapter in [14] Ban GMOs Now, ISIS Report.

To conclude

GMOs are once again found to be deleterious for health in a feeding trial that last no longer than 90 days. And within that time, the most widespread piece of transgenic DNA found in the GM diet, the CaMV 35S promoter, was found transferred horizontally into the animals’ tissues at high frequencies. The CaMV 35S promoter is not the only hazardous piece of transgenic DNA, there are similar aggressive promoters designed to make genes express out of context, as well as genes coding for antibiotics and other dangerous functions, together with numerous recombination hotspots that enhance horizontal gene transfer; all of which contribute to making all GMOs unsafe. That is indeed the conclusion from research carried out by scientists independent of the industry up to now, which fully corroborates what farmers have been witnessing in their livestock and doctors in their patients for years [14]. People need to take immediate action to ban GMOs from their own home and local communities. Governments should recall all GMOs from the market. And companies and regulators should face prosecution for causing damages to health and criminal negligence.